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81.
Shirzad GHOLAMI Farhang BABAMAHMOODI Rohallah ABEDIAN Mehdi SHARIF Abbas SHAHBAZI Abdolsattar PAGHEH Mahdi FAKHAR 《Iranian Journal of Parasitology》2015,10(1):110-115
Background:
Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern areas. The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.Methods:
Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.Results:
A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.Conclusion:
T. colubriformis was the probable most common zoonotic species causing human trichostrongylosis infection in the area 相似文献82.
Mohammad YAKHCHALI Reza MALEKZADEH-VIAYEH Abbas IMANI-BARAN Karim MARDANI 《Iranian Journal of Parasitology》2015,10(1):46-55
Background:
The trematodes of the genus Fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. Differentiation of Fasciola species is important because of their different transmission and epidemiological characteristics. The current study was undertaken to discriminate Fasciola species in the domestic ruminants of Urmia city, Iran.Methods:
Adult flukes were isolated from the naturally infected livers of the slaughtered water buffaloes and sheep. The flukes were initially identified based on morphological and morphometric parameters. A 618-bp-long fragment of the 28SrRNA gene of Fasciola was amplified by polymerase chain reaction (PCR). The amplified fragment was digested by DraII or AvaII enzymes for a restriction fragment length polymorphism (RFLP) analysis and sequenced for the phylogenetic tree construction.Results:
Based on the morphometric examination, the flukes belonged to F. hepatica, F. gigantica and an intermediate Fasciola form. The PCR-RFLP analysis was able to differentiate F. hepatica from F. gigantica. While the phylogenetic reconstruction justified, to some extent, the morphological diagnosis, it failed to segregate F. hepatica from F. gigantica identified in this and the previous studies.Conclusion:
To resolve fully the problem of taxonomy and evolution in Fasciola species, employing a broad range of molecular and morphological approaches is necessary. This is crucial for epidemiological surveys and successful clinical management of their infection. 相似文献83.
Abbas Maleki Afra Khosravi Sobhan Ghafourian Iraj Pakzad Shiva Hosseini Rashid Ramazanzadeh Nourkhoda Sadeghifard 《Osong Public Health and Research Perspectives》2015,6(3):201-204
Objectives
Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aim of this study is to determine the molecular detection of AmpC β-lactamases among clinical Escherichia coli isolated from Ilam hospitals in Ilam, Iran.Methods
One hundred and twelve clinical isolates of E. coli were collected from hospitalized patients and were identified by biochemical tests. They were evaluated for extended spectrum beta-lactamases (ESBLs) production, and the positive strains were subjected to AmpC enzymes; for detection of AmpC cluster genes, multiplex polymerase chain reaction was applied.Results
The analysis showed 62.5% of isolates were ESBLs positive and that five strains revealed the AmpC cluster genes. This is the first report of FOXM cluster genes in E. coli in Iran.Conclusion
Based on our results, the prevalence of AmpC β-lactamases is increasing in Iran, which caused failure in antibiotic therapy. So, the current study recommended the revision of antibiotic policy in Iranian hospitals. 相似文献84.
Agnes I. Berta Abbas Agaimy Joachim M. Braun Bernhard Manger Friedrich E. Kruse Leonard Holbach 《Orbit (Amsterdam, Netherlands)》2015,34(5):299-301
IgG4-related disease is a newly recognized fibro-inflammatory condition. The purpose of this report is to present a patient with 11 years of follow-up, who revealed characteristic features of IgG4-related disease with systemic, orbital and corneal involvement and showed a favorable response to steroids and rituximab treatment. 相似文献
85.
BRCA1 and BRCA2 Mutations in Ethnic Lebanese Arab Women With High Hereditary Risk Breast Cancer 下载免费PDF全文
Nagi S. El Saghir Nathalie K. Zgheib Hussein A. Assi Katia E. Khoury Yannick Bidet Sara M. Jaber Raghid N. Charara Rania A. Farhat Firas Y. Kreidieh Stephanie Decousus Pierre Romero Georges M. Nemer Ziad Salem Ali Shamseddine Arafat Tfayli Jaber Abbas Faek Jamali Muhieddine Seoud Deborah K. Armstrong Yves‐Jean Bignon Nancy Uhrhammer 《The oncologist》2015,20(4):357-364
Purpose.
Breast cancer is the most common malignancy among women in Lebanon and in Arab countries, with 50% of cases presenting before the age of 50 years.Methods.
Between 2009 and 2012, 250 Lebanese women with breast cancer who were considered to be at high risk of carrying BRCA1 or BRCA2 mutations because of presentation at young age and/or positive family history (FH) of breast or ovarian cancer were recruited. Clinical data were analyzed statistically. Coding exons and intron-exon boundaries of BRCA1 and BRCA2 were sequenced from peripheral blood DNA. All patients were tested for BRCA1 rearrangements using multiplex ligation-dependent probe amplification (MLPA). BRCA2 MLPA was done in selected cases.Results.
Overall, 14 of 250 patients (5.6%) carried a deleterious BRCA mutation (7 BRCA1, 7 BRCA2) and 31 (12.4%) carried a variant of uncertain significance. Eight of 74 patients (10.8%) aged ≤40 years with positive FH and only 1 of 74 patients (1.4%) aged ≤40 years without FH had a mutated BRCA. Four of 75 patients (5.3%) aged 41–50 years with FH had a deleterious mutation. Only 1 of 27 patients aged >50 years at diagnosis had a BRCA mutation. All seven patients with BRCA1 mutations had grade 3 infiltrating ductal carcinoma and triple-negative breast cancer. Nine BRCA1 and 17 BRCA2 common haplotypes were observed.Conclusion.
Prevalence of deleterious BRCA mutations is lower than expected and does not support the hypothesis that BRCA mutations alone cause the observed high percentage of breast cancer in young women of Lebanese and Arab descent. Studies to search for other genetic mutations are recommended. 相似文献86.
EZH2 expression is a prognostic factor in childhood intracranial ependymoma: A Canadian Pediatric Brain Tumor Consortium study 下载免费PDF全文
Amanda M. Li MD MSc Christopher Dunham MD Uri Tabori MD Anne‐Sophie Carret MD P. Daniel McNeely MD Donna Johnston MD Lucie Lafay‐Cousin MD Beverly Wilson MD David D. Eisenstat MD MA Nada Jabado MD PhD Shayna Zelcer MD Mariana Silva MD Katrin Scheinemann MD MSc Christopher Fryer MD Glenda Hendson MB BCh Abbas Fotovati PhD Cynthia Hawkins MD PhD Stephen Yip MD PhD Sandra E. Dunn PhD Juliette Hukin MBBS 《Cancer》2015,121(9):1499-1507
87.
Combined DNA methylation and gene expression profiling in gastrointestinal stromal tumors reveals hypomethylation of SPP1 as an independent prognostic factor 下载免费PDF全文
Florian Haller Jitao David Zhang Evgeny A. Moskalev Alexander Braun Claudia Otto Helene Geddert Yasser Riazalhosseini Aoife Ward Aleksandra Balwierz Inga‐Marie Schaefer Silke Cameron B. Michael Ghadimi Abbas Agaimy Jonathan A. Fletcher Jörg Hoheisel Arndt Hartmann Martin Werner Stefan Wiemann Özgür Sahin 《International journal of cancer. Journal international du cancer》2015,136(5):1013-1023
Gastrointestinal stromal tumors (GISTs) have distinct gene expression patterns according to localization, genotype and aggressiveness. DNA methylation at CpG dinucleotides is an important mechanism for regulation of gene expression. We performed targeted DNA methylation analysis of 1.505 CpG loci in 807 cancer‐related genes in a cohort of 76 GISTs, combined with genome‐wide mRNA expression analysis in 22 GISTs, to identify signatures associated with clinicopathological parameters and prognosis. Principal component analysis revealed distinct DNA methylation patterns associated with anatomical localization, genotype, mitotic counts and clinical follow‐up. Methylation of a single CpG dinucleotide in the non‐CpG island promoter of SPP1 was significantly correlated with shorter disease‐free survival. Hypomethylation of this CpG was an independent prognostic parameter in a multivariate analysis compared to anatomical localization, genotype, tumor size and mitotic counts in a cohort of 141 GISTs with clinical follow‐up. The epigenetic regulation of SPP1 was confirmed in vitro, and the functional impact of SPP1 protein on tumorigenesis‐related signaling pathways was demonstrated. In summary, SPP1 promoter methylation is a novel and independent prognostic parameter in GISTs, and might be helpful in estimating the aggressiveness of GISTs from the intermediate‐risk category. 相似文献
88.
89.
90.
Inhibition of microglial activity alters spinal wide dynamic range neuron discharge and reduces microglial Toll‐like receptor 4 expression in neuropathic rats 下载免费PDF全文
Samad Nazemi Homa Manaheji Syyed Mohammad Noorbakhsh Jalal Zaringhalam Mehdi Sadeghi Mohammad Mohammad‐Zadeh Abbas Haghparast 《Clinical and experimental pharmacology & physiology》2015,42(7):772-779
It is believed that neuropathic pain results from aberrant neuronal discharges although some evidence suggests that the activation of glia cells contributes to pain after an injury to the nervous system. This study aimed to evaluate the role of microglial activation on the hyper‐responsiveness of wide dynamic range neurons (WDR) and Toll‐like receptor 4 (TLR4) expressions in a chronic constriction injury (CCI) model of neuropathic pain in rats. Adult male Wistar rats (230 ± 30 g) underwent surgery for induction of CCI neuropathy. Six days after surgery, administration of minocycline (10, 20, and 40 mg/kg, i.p.) was initiated and continued until day 14. After administration of the last dose of minocycline or saline, a behavioral test was conducted, then animals were sacrificed and lumbar segments of the spinal cord were collected for Western blot analysis of TLR4 expression. The electrophysiological properties of WDR neurons were investigated by single unit recordings in separate groups. The findings showed that after CCI, in parallel with thermal hyperalgesia, the expression of TLR4 in the spinal cord and the evoked response of the WDR neurons to electrical, mechanical, and thermal stimulation significantly increased. Post‐injury administration of minocycline effectively decreased thermal hyperalgesia, TLR4 expression, and hyper‐responsiveness of WDR neurons in CCI rats. The results of this study indicate that post‐injury, repeated administration of minocycline attenuated neuropathic pain by suppressing microglia activation and reducing WDR neuron hyper‐responsiveness. This study confirms that post‐injury modulation of microglial activity is a new strategy for treating neuropathic pain. 相似文献